Nigella sativa Seed Oil, branded B’utyQuin™ by TriNutra® Ltd., is a stable cold-pressed black seed oil standardized to 3% thymoquinone and <2% free fatty acids. It is a cosmetic active ingredient that optimizes mitochondrial functions including fat metabolism, ATP production and cell respiration, balances the inflammatory response, and has antioxidant and antimicrobial benefits. These functions were shown in previous research to contribute to relief of seborrhea and dandruff, as well as antiaging and scalp soothing benefits. In this publication, we show how B’utyQuin™ mitigates specific causes of acne in a manner superior to non-standardized black seed oil. Previously, antimicrobial activity against Malassezia furfur, an opportunistic pathogen associated with seborrhea and other dermatological conditions, was shown. This study focuses on B’utyQuin’s ability to significantly inhibit many of the opportunistic pathogens, in particular Propionibacterium acnes, commonly associated with acne and oily skin, in a superior fashion to non-standardized black seed oil. The current study also demonstrates the superiority of B’utyQuin™ compared to non-standardized black seed oil in modulating the microbial diversity present in acne by targeting several key mechanisms of action associated with acne and oily skin.